Chromatography is one of the most popular laboratory techniques. It is a physical method of separation that distributes components to separate between two phases widely used to obtain pure compounds from mixtures. All the chromatography techniques depend on the phase usually finely divided solid or coated solid that a mobile phase, usually a gas or liquid moves through.
What is chromatography used for?
The chromatography is a technique used to separate different biochemical on the basis of their size and is similar to paper chromatography. Chromatography is an impact on your everyday life. The process is used to find out what substances are composed of by separating compounds into their various components and its use affects everything from what you eat and how you fight diseases. Chromatography has gained immense popularity in the past few years in almost every field. Its use is seen in almost every extent around us. It is widely used in the field of chemistry, industry, medicines. It has plentiful applications in the field of biological research. Chromatography is used in airports to detect bombs and is used is forensics in many different ways. It is used to analyze fibers on a person body and also analyze blood found at a crime scene. In gas, chromatography helium is used to move a gaseous mixture through a column of absorbent material.
The three components form the basis of the chromatography technique:
- Stationary phase: This phase is always composed of a solid phase or a layer of a liquid adsorbed on the surface a solid support.
- Mobile phase: This phase is always composed of “liquid” or a “gaseous component.”
- Separated molecules
Types of chromatography:
- Column chromatography
- Ion-exchange chromatography
- Gel-permeation chromatography
- Affinity chromatography
- Paper chromatography
- Thin-layer chromatography
- Gas chromatography
- Dye-ligand chromatography
- Hydrophobic interaction chromatography
- Pseudoaffinity chromatography
- High-pressure liquid chromatography (HPLC)
Example of chromatography:
- It is used in the laboratories for making a pure sample of any compound.
- It is also used to derive the number of reactants or products.
- Percentage purity is also found by chromatography.
- Analytical chemistry use chromatography for various experiments
- The detection and separation of a pure compound are carried by chromatography.